Donkey Chicken IgY conjugated with Agarose
| Code | Size | Price |
|---|
| DAIgY-AGA-5 | 5.0 ml bed volume | £785.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Donkey
Antibody Clonality: Secondary Antibody
Regulatory Status: RUO
Storage:
4°C in the dark. This product has a shelf life of at least 12 months.
Documents
Further Information
Applications Description:
The specific activity of the anti-IgY-agarose was determined by applying 2.7 mg IgY in 24 ml PBS (0.1 mg/ml) to 1.0 ml bed volume of agarose. Two ml aliquots were collected. The total amount of IgY that bound to the column was calculated. For this lot, 1.3 mg IgY bound per ml agarose.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.?
Concentration:
N/A
Formulation:
Phosphate buffered saline, pH 7.2 with 0.075% sodium azide
Product:
Gel; 5 ml settled gel
Product Form:
Immunoprecipitation Gel
Purification Method:
Egg-yolk derived IgY Affinity Purified
Source:
A donkey was immunized with purified chicken IgY. Antibodies were isolated from immune sera using affinity chromatography and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites.
References
1. Hall, Sarah E., Song Luo, Anne E. Hall, and Daphne Preuss, 2005, Differential Rates of Local and Global Homogenization in Centromere Satellites From Arabidopsis Relatives. Genetics: 170(4): 1913?1927.
2. Song, M-G and M. Kiledjian, 2007. 3' Terminal oligo U-tract-mediated stimulation of decapping. RNA, 13:2356-2365.
3. Taylor, A.I., H.J. Gould, B.J. Sutton and R.A. Calvert. 2008. Avian IgY Binds to a Monocyte Receptor with IgG-like Kinetics Despite an IgE-like Structure. J. Biol. Chem. 283: 16384-16390.
4. S-C. Lo and M. Hannink. 2008. PGAM5 tethers a ternary complex containing Keap1 and Nrf2 to mitochondria. Experimental Cell Research 314: 1789-1803.
5. C. Butkinaree, W.D. Cheung, S. Park, K. Park, M. Barber and G.W. Hart, 2008. Characterization of Beta-N-Acetylglucosaminidase Cleavage by Caspase-3 during Apoptosis. J. Biol. Chem., 283:23557-23566.
6. Slawson,C. Lakshmanan, T., Knapp, S. and Hart, G.W., 2008, A Mitotic GlcNAcylation/Phosphorylation Signaling Complex Alters the Posttranslational State of the Cytoskeletal Protein Vimentin. Mol. Biol. Cell 19:4130-4140.
7. McMichael, Brooke K., Cheney, Richard E., and Beth S. Lee. 2010. Myosin X Regulates Sealing Zone Patterning in Osteoclasts through Linkage of Podosomes and Microtubules. J Biol. Chem. 285:9506-9515.
8. Hale, C.R., Sonali, M., Elmore, J., Pfister, N., Compton, M., Olson S., Resch, A.M., Glover III, C.V.C., Graveley B.R., Terns, R.M., and M.P. Terns. 2012. Essential Features and Rational Design of CRISPR RNAs that Function with the Cas RAMP Module Complex to Cleave RNAs. Molecular Cell 45:292-302.
9. Lehman, HL, van Laere SJ, van Golen, CM, Vermeulen PB, Dirix LY and KL van Golen. 2012. Regulation of inflammatory breast cancer cell invasion through Akt1/PKB? phosphorylation of RhoC GTPase. Mol Cancer Res. 10:1306-18.
10. Cheadle L. and T. Biederer. 2012. The novel synaptogenic protein Farp1 links postsynaptic cytoskeletal dynamics and transsynaptic organization. J Cell Biol. 199:985-1001.
11. Dai, L, Taylor, MS, O'Donnell, KA and JD Boeke. 2012. Poly (A) Binding Protein C1 is Essential for Efficient L1 Retrotransposition and Affects L1 RNP Formation. Mol Cell Biol. 32:4323-4336.
12. Joglekar, M, Elbazanti, WO, Weizman, MD, Lehman, HL and van Golen, KL. 2015. Caveolin-1 Mediates Inflammatory Breast Cancer Cell Invasion via the Akt1 Pathway and RhoC GTPase. J. Cell. Biochem 116:923-933.
13. Zhang, Z., Costa, FC, Tan, EP, Bushue, N, DiTacchio, N, Costello, CE, McComb, ME, Whelan, SA, Petersen, KR, and Slawson, C. 2016. O-Linked N-Acetylglucosamine (O-GlcNAc) Transferase and O-GlcNAcase Interact with Mi2? Protein at the A?-Globin Promoter. J. Biol Chem 291:15628-40.
14. Balachandran RS, Heighington CS, Starostina NG, Anderson JW, Owen DL, Vasudevan S, Kipreos ET. 2016. The ubiquitin ligase CRL2ZYG11 targets cyclin B1 for degradation in a conserved pathway that facilitates mitotic slippage. J Cell Biol. 215(2):151-166.
2. Song, M-G and M. Kiledjian, 2007. 3' Terminal oligo U-tract-mediated stimulation of decapping. RNA, 13:2356-2365.
3. Taylor, A.I., H.J. Gould, B.J. Sutton and R.A. Calvert. 2008. Avian IgY Binds to a Monocyte Receptor with IgG-like Kinetics Despite an IgE-like Structure. J. Biol. Chem. 283: 16384-16390.
4. S-C. Lo and M. Hannink. 2008. PGAM5 tethers a ternary complex containing Keap1 and Nrf2 to mitochondria. Experimental Cell Research 314: 1789-1803.
5. C. Butkinaree, W.D. Cheung, S. Park, K. Park, M. Barber and G.W. Hart, 2008. Characterization of Beta-N-Acetylglucosaminidase Cleavage by Caspase-3 during Apoptosis. J. Biol. Chem., 283:23557-23566.
6. Slawson,C. Lakshmanan, T., Knapp, S. and Hart, G.W., 2008, A Mitotic GlcNAcylation/Phosphorylation Signaling Complex Alters the Posttranslational State of the Cytoskeletal Protein Vimentin. Mol. Biol. Cell 19:4130-4140.
7. McMichael, Brooke K., Cheney, Richard E., and Beth S. Lee. 2010. Myosin X Regulates Sealing Zone Patterning in Osteoclasts through Linkage of Podosomes and Microtubules. J Biol. Chem. 285:9506-9515.
8. Hale, C.R., Sonali, M., Elmore, J., Pfister, N., Compton, M., Olson S., Resch, A.M., Glover III, C.V.C., Graveley B.R., Terns, R.M., and M.P. Terns. 2012. Essential Features and Rational Design of CRISPR RNAs that Function with the Cas RAMP Module Complex to Cleave RNAs. Molecular Cell 45:292-302.
9. Lehman, HL, van Laere SJ, van Golen, CM, Vermeulen PB, Dirix LY and KL van Golen. 2012. Regulation of inflammatory breast cancer cell invasion through Akt1/PKB? phosphorylation of RhoC GTPase. Mol Cancer Res. 10:1306-18.
10. Cheadle L. and T. Biederer. 2012. The novel synaptogenic protein Farp1 links postsynaptic cytoskeletal dynamics and transsynaptic organization. J Cell Biol. 199:985-1001.
11. Dai, L, Taylor, MS, O'Donnell, KA and JD Boeke. 2012. Poly (A) Binding Protein C1 is Essential for Efficient L1 Retrotransposition and Affects L1 RNP Formation. Mol Cell Biol. 32:4323-4336.
12. Joglekar, M, Elbazanti, WO, Weizman, MD, Lehman, HL and van Golen, KL. 2015. Caveolin-1 Mediates Inflammatory Breast Cancer Cell Invasion via the Akt1 Pathway and RhoC GTPase. J. Cell. Biochem 116:923-933.
13. Zhang, Z., Costa, FC, Tan, EP, Bushue, N, DiTacchio, N, Costello, CE, McComb, ME, Whelan, SA, Petersen, KR, and Slawson, C. 2016. O-Linked N-Acetylglucosamine (O-GlcNAc) Transferase and O-GlcNAcase Interact with Mi2? Protein at the A?-Globin Promoter. J. Biol Chem 291:15628-40.
14. Balachandran RS, Heighington CS, Starostina NG, Anderson JW, Owen DL, Vasudevan S, Kipreos ET. 2016. The ubiquitin ligase CRL2ZYG11 targets cyclin B1 for degradation in a conserved pathway that facilitates mitotic slippage. J Cell Biol. 215(2):151-166.