AAMP Antibody

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NSJ Bioreagents
Product Code: NSJ-R31545
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31545-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the AAMP antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of AAMP antibody and Lane 1: A431; 2: HeLa; 3: HEPG2; 4: MCF-7. Expected/observed size ~47KD
2 / 5
Western blot testing of AAMP antibody and recombinant human protein (0.5ng)
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IHC-P: AAMP antibody testing of human breast cancer tissue
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Immunofluorescent staining of FFPE human U-2 OS cells with AAMP antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human K562 cells with AAMP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AAMP antibody.
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Western blot testing of AAMP antibody and Lane 1: A431; 2: HeLa; 3: HEPG2; 4: MCF-7. Expected/observed size ~47KD
Western blot testing of AAMP antibody and recombinant human protein (0.5ng)
IHC-P: AAMP antibody testing of human breast cancer tissue
Immunofluorescent staining of FFPE human U-2 OS cells with AAMP antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human K562 cells with AAMP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AAMP antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the AAMP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Angio-associated migratory cell protein is in humans is encoded by the AAMP gene. It is mapped to 2q35. AAMP is an immunoglobulin-type protein which is found to be expressed strongly in endothelial cells, cytotrophoblasts, and poorly differentiated colon adenocarcinoma cells found in lymphatics. It has been demonstrated that an AAMP peptide containing the putative heparan sulfate-binding domain binds to heparin and mediates heparin-sensitive cell adhesion. It plays a role in angiogenesis and cell migration. In smooth muscle cell migration, it may act through the RhoA pathway.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
14
Immunogen:
Human partial recombinant protein (AA 235-434) was used as the immunogen for this AAMP antibody.
Limitation:
This AAMP antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human