ABAT Antibody / 4-aminobutyrate aminotransferase

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NSJ Bioreagents
Product Code: NSJ-R32487
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32487-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the ABAT antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human MCF7 cells with ABAT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HepG2, 2) rat brain, 3) rat liver, 4) mouse brain and 5) mouse liver tissue lysate with ABAT antibody. Predicted molecular weight ~54 kDa.
3 / 3
Flow cytometry testing of human Caco-2 cells with ABAT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ABAT antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with ABAT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HepG2, 2) rat brain, 3) rat liver, 4) mouse brain and 5) mouse liver tissue lysate with ABAT antibody. Predicted molecular weight ~54 kDa.
Flow cytometry testing of human Caco-2 cells with ABAT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ABAT antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
4-Aminobutyrate aminotransferase is a protein that in humans is encoded by the ABAT gene. ABAT is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5- phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of humans ynapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractoryseizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids K388-K500 from the human protein were used as the immunogen for the ABAT antibody.
Limitation:
This ABAT antibody is available for research use only.
Localization:
Cytoplasmic, granular
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P80404