ABCB11 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31844
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31844-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ABCB11 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Western blot testing of 1) rat liver, 2) mouse liver and 3) human SMMC lysate with ABCB11 antibody. Expected/observed molecular weight ~146 kDa.
2 / 6
IHC testing of FFPE human liver cancer with ABCB11 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 6
IHC testing of FFPE mouse liver with ABCB11 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
4 / 6
IHC testing of FFPE rat liver with ABCB11 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
5 / 6
Flow cytometry testing of human A431 cells with ABCB11 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ABCB11 antibody.
6 / 6
Immunofluorescent staining of FFPE mouse liver with ABCB11 antibody (red) and DAPI counterstain (blue). HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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Western blot testing of 1) rat liver, 2) mouse liver and 3) human SMMC lysate with ABCB11 antibody. Expected/observed molecular weight ~146 kDa.
IHC testing of FFPE human liver cancer with ABCB11 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse liver with ABCB11 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat liver with ABCB11 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Flow cytometry testing of human A431 cells with ABCB11 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ABCB11 antibody.
Immunofluorescent staining of FFPE mouse liver with ABCB11 antibody (red) and DAPI counterstain (blue). HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,IF (Paraffin): 1-2ug/ml
Application Note:
Optimal dilution of the ABCB11 antibody should be determined by the researcher.
Description:
Bile Salt Export Pump (BSEP) is a protein which in humans is encoded by the ABCB 11 gene. It is a member of the superfamily of ATP-binding cassette (ABC) transporters, also known as ABCB11. It is mapped to chromosome 2q24. The BSEP protein is mainly expressed in the liver. ABCB11 is a gene associated with progressive familial intrahepatic cholestasis type 2 (PFIC2) which caused by mutations in the ABCB11 gene will increases the risk of hepatocellular carcinoma in early life.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KYGDNTKEIPMERVIAAAKQAQLHD of human ABCB11 were used as the immunogen for the ABCB11 antibody.
Limitation:
This ABCB11 antibody is available for research use only.
Localization:
Membrane
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O95342