ABCB4 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31688
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31688-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
The lyophilized ABCB4 antibody can be stored at 40 to -200. After reconstitution, aliquot and store at -200. Avoid repeated freeze/thaws.

Images

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IHC staining of FFPE human liver cancer with ABCB4 antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.
2 / 3
Western blot testing of 1) human HepG2, 2) rat liver, 3) rat RH35 and 4) mouse liver tissue lysate with ABCB4 antibody. Predicted molecular weight: 135-142 kDa (multiple isoforms).
3 / 3
Flow cytometry testing of human HepG2 cells with ABCB4 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ABCB4 antibody.
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IHC staining of FFPE human liver cancer with ABCB4 antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.
Western blot testing of 1) human HepG2, 2) rat liver, 3) rat RH35 and 4) mouse liver tissue lysate with ABCB4 antibody. Predicted molecular weight: 135-142 kDa (multiple isoforms).
Flow cytometry testing of human HepG2 cells with ABCB4 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ABCB4 antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 2-4ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Variations in secondary/substrate sensitivities and test protocols may require the ABCB4 antibody to be titrated for optimal performance.
Description:
Adenosine triphosphate-binding cassette subfamily B, member 4, also called MDR3, is a protein that in humans is encoded by the ABCB4 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABCB4 mutations represent a genetic factor involved in this peculiar form of cholesterol gallstone disease in adults.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
5244
Immunogen:
Human partial recombinant protein (AA 601-720) was used as the immunogen for this ABCB4 antibody.
Limitation:
This ABCB4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human