ABL2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32426
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32426-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ABL2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC testing of FFPE human gallbladder carcinoma tissue with ABL2 antibody. HEIR: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.
2 / 3
IHC testing of FFPE mouse brain tissue with ABL2 antibody. HEIR: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.
3 / 3
Western blot testing of 1) rat brain, 2) rat PC-12, 3) mouse brain, 4) mouse NIH 3T3 and 5) human K562 cell lysate with ABL2 antibody. Expected molecular weight ~128 kDa, but routinely observed at 128-140 kDa.
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IHC testing of FFPE human gallbladder carcinoma tissue with ABL2 antibody. HEIR: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE mouse brain tissue with ABL2 antibody. HEIR: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.
Western blot testing of 1) rat brain, 2) rat PC-12, 3) mouse brain, 4) mouse NIH 3T3 and 5) human K562 cell lysate with ABL2 antibody. Expected molecular weight ~128 kDa, but routinely observed at 128-140 kDa.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the ABL2 antibody should be determined by the researcher.
Description:
Tyrosine-protein kinase ABL2, also known as Abelson-related gene (Arg), is an enzyme that in humans is encoded by the ABL2 gene. This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids T1065-R1182 of the human protein were used as the immunogen for the ABL2 antibody.
Limitation:
This ABL2 antibody is available for research use only.
Localization:
Cytoplasmic, membranous, nuclear
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
P42684