NSJ Bioreagents

AChR Antibody (muscarinic)

Product Code:
 
NSJ-R31325
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the AChR antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 5
Western blot testing of AChR antibody and lysates from: 1) rat brain, 2) rat C6, 3) mouse RAW246.7, 4) human U-2 OS and 5) human SHG-44 cells. Predicted molecular weight ~51 kDa.
2 / 5
IHC-P: AChR antibody testing of rat brain tissue
3 / 5
IHC staining of FFPE rat brain with AChR antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 5
IF/ICC staining of FFPE human U-2 OS cells with AChR antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 5
IF/ICC staining of FFPE human A431 cells with AChR antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
❮❯

Western blot testing of AChR antibody and lysates from: 1) rat brain, 2) rat C6, 3) mouse RAW246.7, 4) human U-2 OS and 5) human SHG-44 cells. Predicted molecular weight ~51 kDa.
IHC-P: AChR antibody testing of rat brain tissue
IHC staining of FFPE rat brain with AChR antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IF/ICC staining of FFPE human U-2 OS cells with AChR antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IF/ICC staining of FFPE human A431 cells with AChR antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-R31325-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE & frozen): 1-2ug/ml, Immunofluorescence/Immunocytochemistry: 2-4ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the AChR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Muscarinic acetylcholine receptor 1 is a muscarinic receptor. The muscarinic cholinergic receptors belong to a larger family of GPCRs. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. AChR is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 303-317 (KMPMVDPEAQAPTKQ-human) were used as the immunogen for this muscarinic AChR antibody (100% mouse homology).
Limitation:
This AChR antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P11229