NSJ Bioreagents

ACL Antibody / ATP Citrate Lyase

Product Code:
 
NSJ-R32492
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the ATP Citrate Lyase antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 10
IHC testing of FFPE human colon cancer tissue with ATP Citrate Lyase antibody at 2ug/ml. HIER: steam sections in pH8 EDTA for 20 min.
2 / 10
IHC testing of FFPE human colonic adenocarcinoma tissue with ATP Citrate Lyase antibody at 2ug/ml. HIER: steam sections in pH8 EDTA for 20 min.
3 / 10
IHC testing of FFPE human placental tissue with ATP Citrate Lyase antibody at 2ug/ml. HIER: steam sections in pH8 EDTA for 20 min.
4 / 10
IHC testing of FFPE human laryngeal squamous cell carcinoma tissue with ATP Citrate Lyase antibody at 2ug/ml. HIER: steam sections in pH8 EDTA for 20 min.
5 / 10
IHC testing of FFPE human hepatocellular carcinoma tissue with ATP Citrate Lyase antibody at 2ug/ml. HIER: steam sections in pH8 EDTA for 20 min.
6 / 10
IHC testing of FFPE mouse testis tissue with ATP Citrate Lyase antibody at 2ug/ml. HIER: steam sections in pH8 EDTA for 20 min.
7 / 10
IHC testing of FFPE rat testis tissue with ATP Citrate Lyase antibody at 2ug/ml. HIER: steam sections in pH8 EDTA for 20 min.
8 / 10
IF/ICC staining of FFPE human A549 cells with ATP Citrate Lyase antibody (red) at 5ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
9 / 10
Western blot testing of human 1) A549, 2) MOLT4, 3) U-87 MG, 4) U-251, 5) 293T, 6) Hela, 7) T-47D, 8) HepG2, 9) rat pancreas and 10) mouse NIH 3T3 cell lysate with ATP Citrate Lyase antibody at 0.5ug/ml. Predicted molecular weight ~121 kDa.
10 / 10
Flow cytometry testing of human HepG2 cells with ATP Citrate Lyase antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ATP Citrate Lyase antibody.
❮❯

IHC testing of FFPE human colon cancer tissue with ATP Citrate Lyase antibody at 2ug/ml. HIER: steam sections in pH8 EDTA for 20 min.
IHC testing of FFPE human colonic adenocarcinoma tissue with ATP Citrate Lyase antibody at 2ug/ml. HIER: steam sections in pH8 EDTA for 20 min.
IHC testing of FFPE human placental tissue with ATP Citrate Lyase antibody at 2ug/ml. HIER: steam sections in pH8 EDTA for 20 min.
IHC testing of FFPE human laryngeal squamous cell carcinoma tissue with ATP Citrate Lyase antibody at 2ug/ml. HIER: steam sections in pH8 EDTA for 20 min.
IHC testing of FFPE human hepatocellular carcinoma tissue with ATP Citrate Lyase antibody at 2ug/ml. HIER: steam sections in pH8 EDTA for 20 min.
IHC testing of FFPE mouse testis tissue with ATP Citrate Lyase antibody at 2ug/ml. HIER: steam sections in pH8 EDTA for 20 min.
IHC testing of FFPE rat testis tissue with ATP Citrate Lyase antibody at 2ug/ml. HIER: steam sections in pH8 EDTA for 20 min.
IF/ICC staining of FFPE human A549 cells with ATP Citrate Lyase antibody (red) at 5ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) A549, 2) MOLT4, 3) U-87 MG, 4) U-251, 5) 293T, 6) Hela, 7) T-47D, 8) HepG2, 9) rat pancreas and 10) mouse NIH 3T3 cell lysate with ATP Citrate Lyase antibody at 0.5ug/ml. Predicted molecular weight ~121 kDa.
Flow cytometry testing of human HepG2 cells with ATP Citrate Lyase antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ATP Citrate Lyase antibody.

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NSJ-R32492-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml,Immunofluorescence/Immunocytochemistry (FFPE): 5ug/ml,Flow cytometry: 1-3ug/10^6 cells
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
ATP citrate lyase, aslo known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids M1-I180 from the human protein were used as the immunogen for the ATP Citrate Lyase antibody.
Limitation:
This ATP Citrate Lyase antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P53396