NSJ Bioreagents

ACSL1 Antibody / FACL1

Product Code:
 
NSJ-R32493
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the ACSL1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 4
Western blot testing of 1) rat liver, 2) mouse HEPA and 3) A549 lysate with ACSL1 antibody at 0.5ug/ml. Predicted molecular weight ~78 kDa.
2 / 4
IHC testing of FFPE human liver cancer tissue with ACSL1 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
3 / 4
IHC testing of FFPE mouse kidney tissue with ACSL1 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
4 / 4
IHC testing of FFPE rat kidney tissue with ACSL1 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
❮❯

Western blot testing of 1) rat liver, 2) mouse HEPA and 3) A549 lysate with ACSL1 antibody at 0.5ug/ml. Predicted molecular weight ~78 kDa.
IHC testing of FFPE human liver cancer tissue with ACSL1 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
IHC testing of FFPE mouse kidney tissue with ACSL1 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat kidney tissue with ACSL1 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.

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CodeSizePrice
NSJ-R32493-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Long-chain-fatty-acid?CoA ligase 1 is an enzyme that in humans is encoded by the ACSL1 gene. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene. This specific protein is most commonly found in mitochondria and peroxisomes.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids D604-V698 from the human protein were used as the immunogen for the ACSL1 antibody.
Limitation:
This ACSL1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P33121