NSJ Bioreagents

ACTN4 Antibody / Alpha Actinin 4

Product Code:
 
NSJ-R32459
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Prior to reconstitution, store at 40. After reconstitution, the ACTN4 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 6
Western blot testing of 1) rat skeletal muscle, 2) rat liver, 3) rat lung, 4) rat RH35, 5) mouse skeletal muscle, 6) mouse liver, 7) mouse lung and 8) mouse NIH 3T3 cell lysate with ACTN4 antibody at 0.5ug/ml. Predicted molecular weight ~105 kDa.
2 / 6
Western blot testing of human 1) MCF7, 2) HeLa, 3) HepG2, 4) HT1080, 5) placenta, 6) A549, 7) U-251 MG and 8) RT-4 cell lysate with ACTN4 antibody at 0.5ug/ml. Predicted molecular weight ~105 kDa.
3 / 6
IHC staining of FFPE human colonic adenocarcinoma tissue with ACTN4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human prostate cancer tissue with ACTN4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Immunofluorescent staining of FFPE human HeLa cells with ACTN4 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
6 / 6
Flow cytometry testing of human U-87 MG cells with ACTN4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACTN4 antibody.
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Western blot testing of 1) rat skeletal muscle, 2) rat liver, 3) rat lung, 4) rat RH35, 5) mouse skeletal muscle, 6) mouse liver, 7) mouse lung and 8) mouse NIH 3T3 cell lysate with ACTN4 antibody at 0.5ug/ml. Predicted molecular weight ~105 kDa.
Western blot testing of human 1) MCF7, 2) HeLa, 3) HepG2, 4) HT1080, 5) placenta, 6) A549, 7) U-251 MG and 8) RT-4 cell lysate with ACTN4 antibody at 0.5ug/ml. Predicted molecular weight ~105 kDa.
IHC staining of FFPE human colonic adenocarcinoma tissue with ACTN4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer tissue with ACTN4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with ACTN4 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-87 MG cells with ACTN4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACTN4 antibody.

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NSJ-R32459-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Alpha-actinin-4 is a protein that in humans is encoded by the ACTN4 gene. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This ACTN4 gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids E561-V661 from the human protein were used as the immunogen for the ACTN4 antibody.
Limitation:
This ACTN4 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O43707