NSJ Bioreagents

ADAM10 Antibody

Product Code:
 
NSJ-RQ4035
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
After reconstitution, the ADAM10 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of rat 1) thymus and 2) stomach tissue lysate with ADAM10 antibody at 0.5ug/ml. Predicted molecular weight: ~84 kDa (full length), ~60 kDa (active form), ~80 kDa (glycosylated active form), ~110 kDa (glycosylated full length).
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Western blot testing of rat 1) thymus and 2) stomach tissue lysate with ADAM10 antibody at 0.5ug/ml. Predicted molecular weight: ~84 kDa (full length), ~60 kDa (active form), ~80 kDa (glycosylated active form), ~110 kDa (glycosylated full length).

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NSJ-RQ4035-100ug100 ug£535.00
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Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ADAM10 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
ADAM10, A Disintegrin and Metalloproteinase Domain 10, is also known as AD10. ADAM10 is a member of the ADAM family and members of this family are cell surface proteins with a unique structure possessing both potential adhesion and protease function. The ADAM10 gene is mapped to chromosome 15q21.3-q23. ADAM proteins contain an N-terminal signal sequence, followed by a prodomain, a metalloprotease-like domain, a disintegrin-like domain, a cysteine-rich region, an EGF -like repeat, a transmembrane domain, and a C-terminal cytoplasmic tail. Conversion of the membrane-bound precursor to a secreted mature protein is mediated by a protease termed TNFA convertase. ADAM10 possesses TNFA convertase activity.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human partial protein corresponding to amino acids T214-D325 was used as the immunogen for the ADAM10 antibody.
Limitation:
This ADAM10 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O14672