ADAM17 Antibody

Western blot: 0.5-1ug/ml

The stated application concentrations are suggested starting amounts. Titration of the ADAM17 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

ADAM metallopeptidase domain 17, also called TACE (Tumor necrosis factor-alpha-converting enzyme), is an enzyme that belongs to the ADAM proteinfamily of disintegrins and metalloproteases. Expression studies showed that the encoded protein cleaves precursor tumor necrosis factor-alpha to its mature form. Northern blot analysis revealed that the gene was expressed as a 5-kb mRNA in all tissues examined. ADAM17 is understood to be involved in the processing of tumor necrosis factor alpha (TNF-alpha) at the surface of the cell, and from within theintracellular membranesof the trans-Golgi network. This process, which is also known as shedding, involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-alpha), and is of known physiological importance. ADAM17 was the first sheddase to be identified, and is also understood to play a role in the release of a diverse variety of membrane-anchored cytokines, cell adhesion molecules, receptors, ligands, and enzymes.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

An amino acid sequence from the C-terminus of human ADAM17 (AASFKLQRQNRVDSKETEC) was used as the immunogen for this ADAM17 antibody.

This ADAM17 antibody is available for research use only.

Antigen affinity

Human, Mouse, Rat

P78536