Adiponectin Antibody

NSJ Bioreagents
Product Code: NSJ-R31122
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31122-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Adiponectin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Adiponectin antbody and rat heart tissue lysate. Expected/observed size ~30KD
2 / 6
Western blot testing of Adiponectin antbody and recombinant protein; Lane 1: 10ng; 2: 5ng; 3: 2.5ng
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IHC-P: Adiponectin antibody testing of human placenta tissue
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IHC-P: Adiponectin antibody testing of mouse kidney tissue
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IHC-P: Adiponectin antibody testing of rat kidney tissue
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IHC-F testing of Adiponectin antibody and rat liver tissue

Western blot testing of Adiponectin antbody and rat heart tissue lysate. Expected/observed size ~30KD
Western blot testing of Adiponectin antbody and recombinant protein; Lane 1: 10ng; 2: 5ng; 3: 2.5ng
IHC-P: Adiponectin antibody testing of human placenta tissue
IHC-P: Adiponectin antibody testing of mouse kidney tissue
IHC-P: Adiponectin antibody testing of rat kidney tissue
IHC-F testing of Adiponectin antibody and rat liver tissue

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,ELISA: 0.1-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Adiponectin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Adiponectin, als called ADIPOQ (Adipocyte-, C1q-, and Collagen Domain-Containing), APM1, ADPN and ACDC, is a protein which in humans is encoded by the ADIPOQ gene. Using FISH, Das et al.(2001) mapped the mouse Acrp30 gene to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, Schaffler et al.(1999) showed that ADIPOQ is expressed by differentiated adipocytes as a 30-kD protein that is also detectable in serum. By sequence comparisons, they found links between ADIPOQ and TNF family ligands as well as to cytokines expressed by T cells. It is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. It is exclusively secreted from adipose tissue(and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human ADIPOQ (LYADNDNDSTFTGFLLYHDTN) was used as the immunogen for this Adiponectin antibody.
Limitation:
This Adiponectin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q15848