Adiponectin Antibody

NSJ Bioreagents
Product Code: NSJ-R31434
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31434-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Adiponectin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of human 1) placenta, 2) HeLa and 3) Jurkat lysate with Adiponectin antibody at 0.5ug/ml. Predicted molecular weight ~30 kDa.
2 / 4
IHC-P: Adiponectin antibody testing of human placenta tissue. HIER: steamed with pH6 citrate buffer.
3 / 4
IHC-P: Adiponectin antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
4 / 4
IHC-P: Adiponectin antibody testing of human breast cancer tissue. HIER: steamed with pH6 citrate buffer.

Western blot testing of human 1) placenta, 2) HeLa and 3) Jurkat lysate with Adiponectin antibody at 0.5ug/ml. Predicted molecular weight ~30 kDa.
IHC-P: Adiponectin antibody testing of human placenta tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: Adiponectin antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: Adiponectin antibody testing of human breast cancer tissue. HIER: steamed with pH6 citrate buffer.

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Adiponectin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Adiponectin, also known as APM1, ADPN or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. Using FISH, Das et al. (2001) mapped the mouse Acrp30 gene to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, Schaffler et al. (1999) showed that Adiponectin is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, they found links between it and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. Itn is secreted from adipose tissue and from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Human partial recombinant protein (AA 19-244) was used as the immunogen for this Adiponectin antibody.
Limitation:
This Adiponectin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q15848