Adipophilin Antibody / Perilipin 2 / ADRP / PLIN2

NSJ Bioreagents
Product Code: NSJ-R32551
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32551-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Adipophilin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of human MCF7 lysate with ADRP antibody at 0.5ug/ml. Predicted/observed molecular weight ~48 kDa.
2 / 4
IHC testing of FFPE human breast cancer tissue with ADRP antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 4
IHC testing of FFPE mouse testis with ADRP antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
4 / 4
IHC testing of FFPE rat testis with ADRP antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human MCF7 lysate with ADRP antibody at 0.5ug/ml. Predicted/observed molecular weight ~48 kDa.
IHC testing of FFPE human breast cancer tissue with ADRP antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE mouse testis with ADRP antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat testis with ADRP antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Adipose differentiation-related protein, also known as Perilipin 2, PLIN2, ADRP and Adipophilin, is a protein which in humans is encoded by the ADFP gene. The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids K226-Q418 from the human protein were used as the immunogen for the Adipophilin antibody.
Limitation:
This Adipophilin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q99541