ADK Antibody / Adenosine kinase

Contact us
NSJ Bioreagents
Product Code: NSJ-R32499
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32499-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ADK antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Western blot testing of 1) rat liver, 2) rat kidney, 3) rat stomach, 4) rat PC-12, 5) mouse kidney, 6) mouse stomach and 7) mouse NIH 3T3 cell lysate with ADK antibody. Expected molecular weight: 40-45 kDa.
2 / 6
Western blot testing of human 1) placenta, 2) PC-3, 3) T-47D, 4) U937, 5) U-251 MG and 6) HepG2 cell lysate with ADK antibody. Expected molecular weight: 40-45 kDa.
3 / 6
Immunofluorescent staining of FFPE human PC-3 cells with ADK antibody. HIER: steam section in pH6 citrate buffer for 20 min.
4 / 6
IHC staining of FFPE human placenta tissue with ADK antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
IHC staining of FFPE mouse liver tissue with ADK antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 6
IHC staining of FFPE rat liver tissue with ADK antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
❮❯

Western blot testing of 1) rat liver, 2) rat kidney, 3) rat stomach, 4) rat PC-12, 5) mouse kidney, 6) mouse stomach and 7) mouse NIH 3T3 cell lysate with ADK antibody. Expected molecular weight: 40-45 kDa.
Western blot testing of human 1) placenta, 2) PC-3, 3) T-47D, 4) U937, 5) U-251 MG and 6) HepG2 cell lysate with ADK antibody. Expected molecular weight: 40-45 kDa.
Immunofluorescent staining of FFPE human PC-3 cells with ADK antibody. HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human placenta tissue with ADK antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse liver tissue with ADK antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver tissue with ADK antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Adenosine kinase is an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids K165-T351 from the human protein were used as the immunogen for the ADK antibody.
Limitation:
This ADK antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P55263