ADO Antibody / 2-Aminoethanethiol dioxygenase

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NSJ Bioreagents
Product Code: NSJ-R32500
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32500-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the ADO antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Immunofluorescent staining of FFPE human A549 cells with ADO antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 6
Flow cytometry testing of human HeLa cells with ADO antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ADO antibody.
3 / 6
Flow cytometry testing of human A549 cells with ADO antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ADO antibody.
4 / 6
Flow cytometry testing of human U-251 MG cells with ADO antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ADO antibody.
5 / 6
Flow cytometry testing of human PC-3 cells with ADO antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ADO antibody.
6 / 6
Western blot testing of 1) rat brain, 2) rat testis, 3) rat spleen, 4) human MCF-7, 5) human SW620, 6) mouse spleen, 7) mouse heart, 8) mouse HEPA1-6 and 9) mouse SP2/0 lysate with ADO antibody at 0.5ug/ml. Predicted molecular weight ~28/30 kDa.
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Immunofluorescent staining of FFPE human A549 cells with ADO antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human HeLa cells with ADO antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ADO antibody.
Flow cytometry testing of human A549 cells with ADO antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ADO antibody.
Flow cytometry testing of human U-251 MG cells with ADO antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ADO antibody.
Flow cytometry testing of human PC-3 cells with ADO antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ADO antibody.
Western blot testing of 1) rat brain, 2) rat testis, 3) rat spleen, 4) human MCF-7, 5) human SW620, 6) mouse spleen, 7) mouse heart, 8) mouse HEPA1-6 and 9) mouse SP2/0 lysate with ADO antibody at 0.5ug/ml. Predicted molecular weight ~28/30 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids E49-E261 from the human protein were used as the immunogen for the ADO antibody.
Limitation:
This ADO antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q96SZ5