AFP Antibody

NSJ Bioreagents
Product Code: NSJ-R31516
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31516-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the AFP antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human liver cancer tissue with AFP antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.~
2 / 2
Western blot testing of human 1) HepG2 and 2) HCCT cell lysate with AFP antibody.  Pre

IHC staining of FFPE human liver cancer tissue with AFP antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.~
Western blot testing of human 1) HepG2 and 2) HCCT cell lysate with AFP antibody.  Pre

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the AFP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Alpha-Fetoprotein is a protein that in humans is encoded by the AFP gene. It is mapped to 4q13.3. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. In rodents AFP binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. Moreover, it has an important role as a diagnostic marker, especially in certain tumors and liver diseases of childhood. Alpha-Fetoprotein is also used to test the potential usefulness of plasma alpha fetoprotein determination as a diagnostic marker in biliary atresia, hepatitis, and yolk sac derived tumours.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
174
Immunogen:
Human partial recombinant protein (AA 378-609) was used as the immunogen for this AFP antibody.
Limitation:
This AFP antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat