AGFG1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32132
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32132-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the AGFG1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-2 OS cells with AGFG1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human SiHa cells with AGFG1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AGFG1 antibody.
3 / 3
Western blot testing of 1) rat testis, 2) rat liver, 3) human placenta, 4) MCF7 and 5) HeLa lysate with AGFG1 antibody. Expected/observed molecular weight ~58 kDa.
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Immunofluorescent staining of FFPE human U-2 OS cells with AGFG1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human SiHa cells with AGFG1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AGFG1 antibody.
Western blot testing of 1) rat testis, 2) rat liver, 3) human placenta, 4) MCF7 and 5) HeLa lysate with AGFG1 antibody. Expected/observed molecular weight ~58 kDa.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the AGFG1 antibody should be determined by the researcher.
Description:
Arf-GAP domain and FG repeats-containing protein 1 is aprotein that in humans is encoded by the AGFG1 gene. AGFG1 is located to 2q36.3. The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids AKRKQEEKHLKMLRDMTGLPHNRK of human AGFG1 were used as the immunogen for the AGFG1 antibody.
Limitation:
This AGFG1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Rat
Uniprot #:
P52594