AKR1B1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32503
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32503-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the AKR1B1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Flow cytometry testing of human U-2 OS cells with AKR1B1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AKR1B1 antibody.
2 / 5
IHC testing of FFPE human placenta with AKR1B1 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
3 / 5
IHC testing of FFPE rat adrenal gland with AKR1B1 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
4 / 5
IHC testing of FFPE rat intestine with AKR1B1 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
5 / 5
Western blot testing of 1) human SiHa, 2) rat testis and 3) mouse NIH 3T3 cell lysate with AKR1B1 antibody at 0.5ug/ml. Predicted molecular weight ~36 kDa.
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Flow cytometry testing of human U-2 OS cells with AKR1B1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AKR1B1 antibody.
IHC testing of FFPE human placenta with AKR1B1 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat adrenal gland with AKR1B1 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat intestine with AKR1B1 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
Western blot testing of 1) human SiHa, 2) rat testis and 3) mouse NIH 3T3 cell lysate with AKR1B1 antibody at 0.5ug/ml. Predicted molecular weight ~36 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Aldo-keto reductase family 1, member B1 (aldose reductase), also known as AR, is an enzyme that in humans is encoded by the AKR1B1 gene. This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number ofaldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids L228-F316 from the human protein were used as the immunogen for the AKR1B1 antibody.
Limitation:
This AKR1B1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P15121