AKR1C1/2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32504
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32504-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the AKR1C1/2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human HepG2 cells with AKR1C1/2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HepG2, 2) human A549, 3) human HCCP, 4) rat liver, 5) rat kidney, 6) mouse liver and 7) mouse kidney lysate with AKR1C1/2 antibody. Predicted molecular weight ~37 kDa (AKR1C1) and ~37 kDa (AKR1C2).
3 / 3
Flow cytometry testing of human HepG2 cells with AKR1C1/2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AKR1C1/2 antibody.
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Immunofluorescent staining of FFPE human HepG2 cells with AKR1C1/2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HepG2, 2) human A549, 3) human HCCP, 4) rat liver, 5) rat kidney, 6) mouse liver and 7) mouse kidney lysate with AKR1C1/2 antibody. Predicted molecular weight ~37 kDa (AKR1C1) and ~37 kDa (AKR1C2).
Flow cytometry testing of human HepG2 cells with AKR1C1/2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AKR1C1/2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
AKR1C1 and AKR1C2 are members of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. AKR1C1 binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. The AKR1C1 gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids M1-K123 from human AKR1C1 were used as the immunogen for the AKR1C1/2 antibody.
Limitation:
This AKR1C1/2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q04828, P52895