Akt1 Antibody

NSJ Bioreagents
Product Code: NSJ-R30075
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30075-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: PKB-175, aka IML-26
Regulatory Status: RUO
Target Species:
  • Chicken (Gallus)
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the Akt1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Akt1 antibody and Lane 1: rat ovary; 2: PC-12; 3: A549; 4: MCF-7; 5: HEPA; Predicted size: 56KD; Observed size: 56KD

Western blot testing of Akt1 antibody and Lane 1: rat ovary; 2: PC-12; 3: A549; 4: MCF-7; 5: HEPA; Predicted size: 56KD; Observed size: 56KD

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Akt1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PKB alpha also knows as V-AKT murine thymoma vial oncogene homolog 1(ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. The gene is mapped to chromosome 14q32.3. The protein is critical for ischemic and VEGF-mediated angiogenesis. It regulates pathological angiogenesis, vascular maturation and permeability in vivo.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
207
Immunogen:
Amino acids 461-477 of human PKB alpha/Akt1 were used as the immunogen for this Akt1 antibody.
Limitation:
This Akt1 antibody is available for research use only.
Purity:
Ascites
Species Reactivity :
Human, mouse, rat, chicken