AKT2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31474
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31474-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the AKT2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of AKT2 antibody and Lane 1: HeLa; 2: PANC; 3: A549; 4: COLO320; 5: HT1080; 6: MCF-7 lysate
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IHC-P: AKT2 antibody testing of human intestine cancer tissue
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IHC-P: AKT2 antibody testing of human lung cancer tissue
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Western blot testing of AKT2 antibody and Lane 1: HeLa; 2: PANC; 3: A549; 4: COLO320; 5: HT1080; 6: MCF-7 lysate
IHC-P: AKT2 antibody testing of human intestine cancer tissue
IHC-P: AKT2 antibody testing of human lung cancer tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the AKT2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. This gene is mapped to 19q13.2. AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, -2 and -3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. It is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
208
Immunogen:
An amino acid sequence from the C-terminus of human AKT2 (DRYDSLGLLELDQRTHFPQFSYSASIRE) was used as the immunogen for this AKT2 antibody.
Limitation:
This AKT2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat