Alcohol dehydrogenase Antibody / ADH1A

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NSJ Bioreagents
Product Code: NSJ-R32497
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32497-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Alcohol dehydrogenase antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-2 OS cells with Alcohol dehydrogenase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 8
Immunofluorescent staining of FFPE human U-2 OS cells with Alcohol dehydrogenase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC testing of FFPE human liver cancer tissue with Alcohol dehydrogenase antibody. HIER: steam sections in pH6 citrate buffer for 20 min.
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IHC testing of FFPE human liver cancer tissue with Alcohol dehydrogenase antibody. HIER: steam sections in pH8 EDTA buffer for 20 min.
5 / 8
IHC testing of FFPE mouse liver tissue with Alcohol dehydrogenase antibody. HIER: steam sections in pH8 EDTA buffer for 20 min.
6 / 8
IHC testing of FFPE rat liver tissue with Alcohol dehydrogenase antibody. HIER: steam sections in pH8 EDTA buffer for 20 min.
7 / 8
Western blot testing of 1) human HCCT, 2) human HCCP, 3) rat liver, 4) rat kidney, 5) mouse liver and 6) mouse kidney lysate with Alcohol dehydrogenase antibody at 0.5ug/ml. Predicted molecular weight ~40 kDa.
8 / 8
Flow cytometry testing of human U-2 OS cells with Alcohol dehydrogenase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Alcohol dehydrogenase antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with Alcohol dehydrogenase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with Alcohol dehydrogenase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human liver cancer tissue with Alcohol dehydrogenase antibody. HIER: steam sections in pH6 citrate buffer for 20 min.
IHC testing of FFPE human liver cancer tissue with Alcohol dehydrogenase antibody. HIER: steam sections in pH8 EDTA buffer for 20 min.
IHC testing of FFPE mouse liver tissue with Alcohol dehydrogenase antibody. HIER: steam sections in pH8 EDTA buffer for 20 min.
IHC testing of FFPE rat liver tissue with Alcohol dehydrogenase antibody. HIER: steam sections in pH8 EDTA buffer for 20 min.
Western blot testing of 1) human HCCT, 2) human HCCP, 3) rat liver, 4) rat kidney, 5) mouse liver and 6) mouse kidney lysate with Alcohol dehydrogenase antibody at 0.5ug/ml. Predicted molecular weight ~40 kDa.
Flow cytometry testing of human U-2 OS cells with Alcohol dehydrogenase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Alcohol dehydrogenase antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Alcohol dehydrogenase 1A is an enzyme that in humans is encoded by the ADH1A gene. This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. And this gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids K213-F375 from human ADH1A were used as the immunogen for the Alcohol dehydrogenase antibody.
Limitation:
This Alcohol dehydrogenase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P07327