ALDH1A2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32466
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32466-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
Prior to reconstitution, store at 40. After reconstitution, the ALDH1A2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human colorectal adenocarcinoma tissue with ALDH1A2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE rat lung tissue with ALDH1A2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Western blot testing of 1) human K562, 2) human HepG2, 3) rat lung, 4) rat heart, 5) rat kidney, 6) mouse lung, 7) mouse kidney and 8) mouse ovary with ALDH1A2 antibody. Predicted molecular weight ~56 kDa.
4 / 5
Western blot testing of 1) human K562, 2) human 293T, 3) human HepG2, 4) human HeLa, 5) rat kidney, 6) rat testis, 7) mouse kidney and 8) mouse testis with ALDH1A2 antibody. Predicted molecular weight ~56 kDa.
5 / 5
Flow cytometry testing of human 293T cells with ALDH1A2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ALDH1A2 antibody.
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IHC staining of FFPE human colorectal adenocarcinoma tissue with ALDH1A2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat lung tissue with ALDH1A2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human K562, 2) human HepG2, 3) rat lung, 4) rat heart, 5) rat kidney, 6) mouse lung, 7) mouse kidney and 8) mouse ovary with ALDH1A2 antibody. Predicted molecular weight ~56 kDa.
Western blot testing of 1) human K562, 2) human 293T, 3) human HepG2, 4) human HeLa, 5) rat kidney, 6) rat testis, 7) mouse kidney and 8) mouse testis with ALDH1A2 antibody. Predicted molecular weight ~56 kDa.
Flow cytometry testing of human 293T cells with ALDH1A2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ALDH1A2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Aldehyde dehydrogenase 1 family, member A2, also known as ALDH1A2 or retinaldehyde dehydrogenase 2 (RALDH2), is an enzyme that in humans is encoded by the ALDH1A2 gene. This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Four transcript variants encoding distinct isoforms have been identified for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids M1-A110 from the human protein were used as the immunogen for the ALDH1A2 antibody.
Limitation:
This ALDH1A2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O94788