ALDH2 Antibody

Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml

The stated application concentrations are suggested starting amounts. Titration of the ALDH2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Aldehyde Dehydrogenase 2 is a human gene. The enzyme encoded by this gene belongs to the ALDH family of enzymes that catalyze the chemical transformation from acetaldehyde to acetic acid. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Hsu et al.(1985) assigned the ALDH2 locus to chromosome 12 by means of a cDNA probe and Southern blot analysis of somatic cell hybrids. Using an unbiased proteomic search, Chen et al.(2008) identified mitochondrial ALDH2 as an enzyme whose activation correlated with reduced ischemic heart damage in rodent models. A high-throughput screen identified a small molecule activator of ALDH2, which they called Alda-1, that, when administered to rats before an ischemic event, reduced infarct size by 60%, most likely through its inhibitory effect on the formation of cytotoxic aldehydes.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

An amino acid sequence from the N-terminus of human Aldehyde Dehydrogenase 2 (SAAATQAVPAPNQQPE) was used as the immunogen for this ALDH2 antibody.

This ALDH2 antibody is available for research use only.

Antigen affinity

Human, Rat

P05091