ALK Antibody

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NSJ Bioreagents
Product Code: NSJ-R30831
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30831-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ALK antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE human rectal cancer with ALK antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE rat intestine with ALK antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
3 / 3
Western blot testing of ALK antibody and Lane 1: rat brain; 2: rat testis; 3: human U87; 4: HeLa; 5: COLO320; 6: Jurkat cell lysate. Predicted molecular weight: 190-220 kDa
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IHC staining of FFPE human rectal cancer with ALK antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with ALK antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
Western blot testing of ALK antibody and Lane 1: rat brain; 2: rat testis; 3: human U87; 4: HeLa; 5: COLO320; 6: Jurkat cell lysate. Predicted molecular weight: 190-220 kDa

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the ALK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Anaplastic lymphoma kinase also known as CD246 (cluster of differentiation 246), is an enzyme that in humans is encoded by the ALK gene. Expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. The deduced amino acid sequences reveal that ALK is a novel receptor tyrosine kinase having a putative transmembrane domain and an extracellular domain. In Drosophila, localized Jeb activates Alk and the downstream Ras/mitogen-activated protein kinase cascade to specify a select group of visceral muscle precursors as muscle-patterning pioneers. Functional RNA interference screening on a set of these transcriptional targets revealed that CEBPB and BCL2A1 were absolutely necessary to induce cell transformation and/or to sustain growth and survival of ALK-positive ALCL cells. One particularly informative case presented a high-level gene amplification that was strictly limited to ALK, indicating that this gene may contribute on its own to neuroblastoma development. Mutated ALK proteins were overexpressed, hyperphosphorylated, and showed constitutive kinase activity. The knockdown of expression in ALK-mutated cells, but also in cell lines overexpressing the wildtype protein, led to a marked decrease of cell proliferation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human ALK (LFRLRHFPCGNVNYGYQQQ) was used as the immunogen for this ALK antibody (100% homologous in human, mouse and rat).
Limitation:
This ALK antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UM73