Alkaline phosphatase Antibody (placental)

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NSJ Bioreagents
Product Code: NSJ-R32610
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32610-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PLAP antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE human HeLa cells with PLAP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC testing of FFPE human placental tissue with PLAP antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 5
Western blot testing of human placenta lysate with PLAP antibody. Predicted molecular weight ~58 kDa but routinely visualized at 60-70 kDa.
4 / 5
Flow cytometry testing of human SiHa cells with PLAP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PLAP antibody.
5 / 5
Immunofluorescent staining of FFPE human placental tissue with PLAP antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with PLAP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human placental tissue with PLAP antibody. HIER: steam section in pH8 EDTA buffer for 20 min.
Western blot testing of human placenta lysate with PLAP antibody. Predicted molecular weight ~58 kDa but routinely visualized at 60-70 kDa.
Flow cytometry testing of human SiHa cells with PLAP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PLAP antibody.
Immunofluorescent staining of FFPE human placental tissue with PLAP antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the PLAP antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Alkaline phosphatase, placental type also known as placental alkaline phosphatase (PLAP) is an allosteric enzyme that in humans is encoded by the ALPP gene. The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 403-433 (KARDRKAYTVLLYGNGPGYVLKDGARPDVTE-human) were used as the immunogen for the PLAP antibody.
Limitation:
This antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P05187