Alpha Catenin Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R31558
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31558-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the alpha Catenin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of alpha Catenin antibody and Lane 1: rat liver; 2: (r) lung; 3: (r) heart; 4: mouse NIH3T3; 5: (r) PC12; 6: human HEPG2; 7: (h) HeLa; 8: (h) MCF-7; 9: (m) HEPA. Expected/observed size ~102KD
2 / 5
Western blot testing of alpha Catenin antibody and recombinant human protein (0.5ng)
3 / 5
IHC-P: alpha Catenin antibody testing of human breast tissue
4 / 5
IHC-P: alpha Catenin antibody testing of mouse intestine tissue
5 / 5
IHC-P staining of rat intestine tissue
❮❯

Western blot testing of alpha Catenin antibody and Lane 1: rat liver; 2: (r) lung; 3: (r) heart; 4: mouse NIH3T3; 5: (r) PC12; 6: human HEPG2; 7: (h) HeLa; 8: (h) MCF-7; 9: (m) HEPA. Expected/observed size ~102KD
Western blot testing of alpha Catenin antibody and recombinant human protein (0.5ng)
IHC-P: alpha Catenin antibody testing of human breast tissue
IHC-P: alpha Catenin antibody testing of mouse intestine tissue
IHC-P staining of rat intestine tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the alpha Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Alpha Catenin is a protein that in humans is encoded by the CTNNA1 gene. When surface epithelium CTNNA1 was ablated, hair follicle development was blocked and epidermal morphogenesis was dramatically affected, with defects in adherens junction formation, intercellular adhesion, and epithelial polarity. In vitro, CTNNA1 null keratinocytes were poorly contact inhibited and grew rapidly. These differences were not dependent upon intercellular adhesion and were in marked contrast to keratinocytes conditionally null for another essential intercellular adhesion protein, desmoplakin Knockout keratinocytes exhibited sustained activation of the Ras-MAPK cascade due to aberrations in growth factor responses. It is concluded that features of precancerous lesions often attributed to defects in cell cycle regulatory genes can be generated by compromising the function of CTNNA1.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
1495
Immunogen:
Human partial recombinant protein (AA 143-292) was used as the immunogen for this alpha Catenin antibody.
Limitation:
This alpha Catenin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat