Alpha Internexin Antibody / Neurofilament-66

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NSJ Bioreagents
Product Code: NSJ-R32440
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32440-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Prior to reconstitution, store at 40. After reconstitution, the Alpha Internexin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of 1) rat brain, 2) mouse brain and 3) human 22RV1 lysate with Alpha Internexin antibody at 0.5ug/ml. Predicted molecular weight ~55 kDa but routinely observed at ~66 kDa.
2 / 4
IHC testing of FFPE human glioma tissue with HMGB2 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
3 / 4
IHC testing of FFPE mouse brain with HMGB2 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
4 / 4
IHC testing of FFPE rat brain with HMGB2 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
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Western blot testing of 1) rat brain, 2) mouse brain and 3) human 22RV1 lysate with Alpha Internexin antibody at 0.5ug/ml. Predicted molecular weight ~55 kDa but routinely observed at ~66 kDa.
IHC testing of FFPE human glioma tissue with HMGB2 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
IHC testing of FFPE mouse brain with HMGB2 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
IHC testing of FFPE rat brain with HMGB2 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Alpha-Internexin (INA; also NF-66) is a 66 kDa member of the intermediate filament (IF) protein family. The protein was originally purified from rat optic nerve and spinal cord. And the protein copurifies with other neurofilament subunits, as it was originally discovered, however in some mature neurons it can be the only neurofilament expressed. The protein is present in developing neuroblasts and in the Central Nervous System of adults. Meanwhile, the protein is a major component of the intermediate filament network in small interneurons and cerebellar granule cells, where it is present in the parallel fibers.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids A71-R161 were used as the immunogen for the Alpha Internexin antibody.
Limitation:
This Alpha Internexin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q16352