Alpha Tubulin Antibody

NSJ Bioreagents
Product Code: NSJ-R30095
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30095-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: DM1A, aka Tub-1
Regulatory Status: RUO
Target Species:
  • Chicken (Gallus)
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Alpha Tubulin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human SH-SY5Y, 2) human Jurkat, 3) human A549, 4) rat brain, 5) mouse brain and 6) mouse thymus tissue lysate with Alpha Tubulin antibody. Predicted molecular weight ~50 kDa.~
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IHC-P: Alpha Tubulin antibody testing of hum

Western blot testing of 1) human SH-SY5Y, 2) human Jurkat, 3) human A549, 4) rat brain, 5) mouse brain and 6) mouse thymus tissue lysate with Alpha Tubulin antibody. Predicted molecular weight ~50 kDa.~
IHC-P: Alpha Tubulin antibody testing of hum

Further Information

Application Details :
Western blot: 2ug/ml,IHC (Paraffin): 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the Alpha Tubulin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Alpha-tubulin (b-alpha-1) mRNA is expressed at a molecular weight of about 55,000D. The 3-prime UTR of the protein is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene IL-alpha-T1. Alpha tubulin is a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Immunogen:
Microtubules from chicken embryo brain was used as the immunogen for this Alpha Tubulin antibody.
Limitation:
This Alpha Tubulin antibody is available for research use only.
Purity:
Unpurified ascites
Species Reactivity :
Human, mouse, rat, chicken
Uniprot #:
P68366