Alpha-Actinin Antibody

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NSJ Bioreagents
Product Code: NSJ-R30092
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30092-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: EA-53, aka SA-20
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rabbit
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the alpha-Actinin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of 1) rat heart, 2) rat skeletal muscle, 3) mouse heart, 4) mouse skeletal muscle and 5) human placenta lysate with alpha-Actinin antibody at 0.5ug/ml. Predicted molecular weight ~105 kDa.
2 / 4
Western blot testing of 1) mouse heart, 2) mouse skeletal muscle, 3) rat heart and 4) rat skeletal muscle lysate with alpha-Actinin antibody at 1ug/ml. Predicted molecular weight ~105 kDa.
3 / 4
IHC testing of FFPE human breast cancer tissue with alpha-Actinin antibody at 1.5ug/ml. Required HIER: boil the sections in 10mM Citrate buffer, pH6, and allow to cool prior to staining.
4 / 4
IHC testing of FFPE mouse lung tissue with alpha-Actinin antibody at 1.5 ug/ml. Required HIER: boil the sections in 10mM Citrate buffer, pH6, and allow to cool prior to staining.
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Western blot testing of 1) rat heart, 2) rat skeletal muscle, 3) mouse heart, 4) mouse skeletal muscle and 5) human placenta lysate with alpha-Actinin antibody at 0.5ug/ml. Predicted molecular weight ~105 kDa.
Western blot testing of 1) mouse heart, 2) mouse skeletal muscle, 3) rat heart and 4) rat skeletal muscle lysate with alpha-Actinin antibody at 1ug/ml. Predicted molecular weight ~105 kDa.
IHC testing of FFPE human breast cancer tissue with alpha-Actinin antibody at 1.5ug/ml. Required HIER: boil the sections in 10mM Citrate buffer, pH6, and allow to cool prior to staining.
IHC testing of FFPE mouse lung tissue with alpha-Actinin antibody at 1.5 ug/ml. Required HIER: boil the sections in 10mM Citrate buffer, pH6, and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 3-5ug/ml,IHC (Paraffin): 4-7ug/ml (1),Immunohistochemistry (Frozen): 4-7ug/ml to detect in formalin or acetone fixed tissues
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the alpha-Actinin antibody may be required due to differences in protocols and secondary/substrate sensitivity. 1. Boiling the sections in 10mM Citrate buffer, pH6, is a REQUIRED pretreatment
Description:
Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin(alpha A) shares structural homology with spectrin and dystrophin.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
88
Immunogen:
Rabbit skeletal alpha-actinin was used as the immunogen for this alpha-Actinin antibody.
Limitation:
This alpha-Actinin antibody is available for research use only.
Purity:
Ascites
Species Reactivity :
Human, mouse, rat, rabbit