Alpha-Adaptin Antibody

NSJ Bioreagents
Product Code: NSJ-R30093
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30093-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 100/2, aka Ada-1
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the alpha-Adaptin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human SH-SY5Y, 2) human U-87 MG, 3) human MCF7, 4) human 293T, 5) rat brain, 6) rat C6 and 7) mouse brain tissue lysate with Alpha-Adaptin antibody. Predicted molecular weight ~108 kDa.

Western blot testing of 1) human SH-SY5Y, 2) human U-87 MG, 3) human MCF7, 4) human 293T, 5) rat brain, 6) rat C6 and 7) mouse brain tissue lysate with Alpha-Adaptin antibody. Predicted molecular weight ~108 kDa.

Further Information

Application Details :
Western blot: 2-4ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the alpha-Adaptin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1(HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al.(2001) identified 3 HIP1-associated proteins, clathrin heavy chain(CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD(adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
160
Immunogen:
AP-2 adaptor polypeptides from bovine brain was used as the immunogen for this alpha-Adaptin antibody.
Limitation:
This alpha-Adaptin antibody is available for research use only.
Purity:
Ascites
Species Reactivity :
Human, rat