AMFR Antibody / GP78

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NSJ Bioreagents
Product Code: NSJ-R32507
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32507-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the GP78 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
Immunofluorescent staining of FFPE human T-47D cells with GP78 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 7
IHC testing of FFPE human placenta with GP78 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
3 / 7
IHC staining of FFPE mouse intestine with GP78 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE rat kidney with GP78 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 7
Flow cytometry testing of human U-87 MG cells with GP78 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GP78 antibody.
6 / 7
Flow cytometry testing of human SiHa cells with GP78 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GP78 antibody.
7 / 7
Western blot testing of 1) human HepG2, 2) human HeLa, 3) human Daudi and 4) rat kidney lysate with GP78 antibody at 0.5ug/ml. Expected molecular weight 73-78 kDa.
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Immunofluorescent staining of FFPE human T-47D cells with GP78 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human placenta with GP78 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
IHC staining of FFPE mouse intestine with GP78 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney with GP78 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human U-87 MG cells with GP78 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GP78 antibody.
Flow cytometry testing of human SiHa cells with GP78 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GP78 antibody.
Western blot testing of 1) human HepG2, 2) human HeLa, 3) human Daudi and 4) rat kidney lysate with GP78 antibody at 0.5ug/ml. Expected molecular weight 73-78 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Autocrine motility factor receptor, isoform 2, also called GP78, is a protein that in humans is encoded by the AMFR gene. Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids E553-S643 from the human protein were used as the immunogen for the GP78 antibody.
Limitation:
This GP78 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UKV5