Amyloid beta Antibody / APP

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NSJ Bioreagents
Product Code: NSJ-R31517
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31517-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Amyloid beta antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa, 2) U-87 MG, 3) T-47D, 4) A549, 5) U-2 OS, 6) rat brain and 7) mouse brain lysate with Amyloid beta antibody. Predicted molecular weight 79~120 kDa depending on glycosylation level.
2 / 7
IF/ICC staining of FFPE human A431 cells with Amyloid beta antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE human glioma tissue with Amyloid beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE mouse brain with Amyloid beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE rat brain with Amyloid beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
6 / 7
IHC staining of FFPE human renal cancer with Amyloid beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
7 / 7
IHC staining of FFPE human tonsil with Amyloid beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of human 1) HeLa, 2) U-87 MG, 3) T-47D, 4) A549, 5) U-2 OS, 6) rat brain and 7) mouse brain lysate with Amyloid beta antibody. Predicted molecular weight 79~120 kDa depending on glycosylation level.
IF/ICC staining of FFPE human A431 cells with Amyloid beta antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human glioma tissue with Amyloid beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with Amyloid beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat brain with Amyloid beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human renal cancer with Amyloid beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with Amyloid beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence/Immunocytochemistry: 2-4ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Amyloid beta antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Amyloid beta, also called Abeta and APP, denotes peptides that are crucially involved in Alzheimers disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. Several potential activities have been discovered for Amyloid beta, including activation of kinase enzymes, functioning as a transcription factor, and anti-microbial activity (potentially associated with it pro-inflammatory activity). Moreover, monomeric Amyloid beta is indicated to protect neurons by quenching metal-inducible oxygen radical generation and thereby inhibiting neurotoxicity.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
351
Immunogen:
An amino acid sequence from the C-terminus of human APP (DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA) was used as the immunogen for this Amyloid beta antibody.
Limitation:
This Amyloid beta antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P05067