Angiostatin K1-3 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30129
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30129-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Angiostatin K1-3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) SMMC-7721 and 2) HepG2 cell lysate with Angiostatin K1-3 antibody. Expected molecular weight of Plasminogen: 90-95 kDa.
2 / 3
IHC staining of FFPE human placenta with Angiostatin K1-3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 3
IHC staining of FFPE human breast cancer with Angiostatin K1-3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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Western blot testing of human 1) SMMC-7721 and 2) HepG2 cell lysate with Angiostatin K1-3 antibody. Expected molecular weight of Plasminogen: 90-95 kDa.
IHC staining of FFPE human placenta with Angiostatin K1-3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with Angiostatin K1-3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,ELISA: 0.1-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Angiostatin K1-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity(ED50=70nM) relative to kringles 1-4(ED50 = 135nM).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Human partial recombinant protein (AA 103-352) was used as the immunogen for this Angiostatin K1-3 antibody.
Limitation:
This Angiostatin K1-3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P00747