Angiotensin II Type 1 Receptor Antibody (AGTR1, AT1R)

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NSJ Bioreagents
Product Code: NSJ-R32059
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32059-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the AT1R antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Western blot testing of 1) HeLa and 2) HEPA cell lysate with AT1R antibody. Observed molecular weight ~41-50 kDa depending on glycosylation level.
2 / 3
IHC testing of FFPE human breast cancer tissue with AT1R antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 3
Western blot testing of 1) human HEK293, 2) human T-47D, 3) human PC-3, 4) human U-87 MG, 5) human HepG2, 6) monkey COS-7, 7) rat brain, 8) rat liver, 9) rat kidney, 10) rat RH35, 11) mouse brain, 12) mouse liver, 13) mouse kidney and 14) mouse HEPA1-6 lysate with AT1R antibody. Observed molecular weight ~41-50 kDa depending on glycosylation level.
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Western blot testing of 1) HeLa and 2) HEPA cell lysate with AT1R antibody. Observed molecular weight ~41-50 kDa depending on glycosylation level.
IHC testing of FFPE human breast cancer tissue with AT1R antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Western blot testing of 1) human HEK293, 2) human T-47D, 3) human PC-3, 4) human U-87 MG, 5) human HepG2, 6) monkey COS-7, 7) rat brain, 8) rat liver, 9) rat kidney, 10) rat RH35, 11) mouse brain, 12) mouse liver, 13) mouse kidney and 14) mouse HEPA1-6 lysate with AT1R antibody. Observed molecular weight ~41-50 kDa depending on glycosylation level.

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Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
Optimal dilution of the AT1R antibody should be determined by the researcher.
Description:
AGTR1, or AT1R, is known as Angiotensin II Type 1 Receptor. Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. And it acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. Moreover, this gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids MILNSSTEDGIKRIQDDCPKAGRHNYIF of human AGTR1 were used as the immunogen for the AT1R antibody.
Limitation:
This AT1R antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
P30556