Annexin A1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30173
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30173-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Annexin A1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A431 cells with Annexin A1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC-P: Annexin A1 antibody testing of human placenta tissue
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IHC-P: Annexin A1 antibody testing of human tonsil tissue
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Western blot testing of 1) human HeLa, 2) human K562 and 3) monkey COS-7 cell lysate with Annexin A1 antibody. Predicted molecular weight ~38 kDa.
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Flow cytometry testing of human A431 cells with Annexin A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Annexin A1 antibody.
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Immunofluorescent staining of FFPE human A431 cells with Annexin A1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: Annexin A1 antibody testing of human placenta tissue
IHC-P: Annexin A1 antibody testing of human tonsil tissue
Western blot testing of 1) human HeLa, 2) human K562 and 3) monkey COS-7 cell lysate with Annexin A1 antibody. Predicted molecular weight ~38 kDa.
Flow cytometry testing of human A431 cells with Annexin A1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Annexin A1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Annexin A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Annexin I, also known as lipocortin I(Lipo1), belongs to the family of annexins. These proteins are though to control the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes. In two lipocortins(I and II) a short amino-terminal sequence distinct from the core structure has potential regulatory functions which are dependent on its phosphorylation state. The gene in the mouse encodes a protein of 346 amino acid residues. Mouse Lipo1 gene spans about 17 kb and is divided into 13 exons. Annexin I gene, mapped to 9q11-q22, is located on mouse chromosome 19. Annexin I acts through the formyl peptide receptor on human neutrophils. Peptides derived from the unique N-terminal domain of annexin I serve as FPR ligands and trigger different signaling pathways in a dose-dependent manner.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human Annexin A1 (MVSEFLKQAWFIENEEQEYVQT) was used as the immunogen for this Annexin A1 antibody.
Limitation:
This Annexin A1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P04083