Annexin A3 Antibody

NSJ Bioreagents
Product Code: NSJ-R31948
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31948-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Annexin A3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
Western blot testing of 1) rat spleen, 2) rat stomach, 3) human HeLa, 4) 22RV1, 5) A549, 6) MCF7 and 7) SW620 antibody with Annexin A3 antibody. Predicted/observed molecular weight ~36 kDa.
2 / 7
IHC testing of FFPE human breast cancer with Annexin A3 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 7
IHC testing of FFPE mouse heart with Annexin A3 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
4 / 7
IHC testing of FFPE rat heart with Annexin A3 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
5 / 7
IHC testing of frozen human placenta with Annexin A3 antibody.
6 / 7
IHC testing of frozen mouse heart with Annexin A3 antibody.
7 / 7
IHC testing of frozen rat heart with Annexin A3 antibody.

Western blot testing of 1) rat spleen, 2) rat stomach, 3) human HeLa, 4) 22RV1, 5) A549, 6) MCF7 and 7) SW620 antibody with Annexin A3 antibody. Predicted/observed molecular weight ~36 kDa.
IHC testing of FFPE human breast cancer with Annexin A3 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse heart with Annexin A3 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat heart with Annexin A3 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of frozen human placenta with Annexin A3 antibody.
IHC testing of frozen mouse heart with Annexin A3 antibody.
IHC testing of frozen rat heart with Annexin A3 antibody.

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml
Application Note:
Optimal dilution of the Annexin A3 antibody should be determined by the researcher.
Description:
Annexin A3 is a protein that in humans is encoded by the Annexin A3 gene. The Annexin A3 gene contains 13 exons and spans 58 kb of genomic DNA. The Annexin A3 gene is mapped to 4q21. It is abnormally expressed in fetuses of both IVF and ICSI, which may contribute to the increase risk of birth defects in these ART. This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phospholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids RKALLTLADGRRDESLKVDEHLAKQDAQILYK of human Annexin A3 were used as the immunogen for the Annexin A3 antibody.
Limitation:
This Annexin A3 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P12429