Annexin V Antibody

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NSJ Bioreagents
Product Code: NSJ-R30176
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30176-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Annexin V antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Annexin V antibody and Lane 1: rat brain; 2: rat skeletal muscle; 3: rat ovary; 4: rat lung; 5: MCF-7; 6: SMMC-7721; 7: A549; 8: Jurkat; 9: SGC; 10: HT1080; Predicted molecular weight ~36 kDa.
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IHC-P: Annexin V antibody testing of rat liver tissue
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ICC: Annexin V antibody testing of HCT116 cells
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ICC testing of HeLa cells
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Western blot testing of Annexin V antibody and Lane 1: rat brain; 2: rat skeletal muscle; 3: rat ovary; 4: rat lung; 5: MCF-7; 6: SMMC-7721; 7: A549; 8: Jurkat; 9: SGC; 10: HT1080; Predicted molecular weight ~36 kDa.
IHC-P: Annexin V antibody testing of rat liver tissue
ICC: Annexin V antibody testing of HCT116 cells
ICC testing of HeLa cells

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Annexin V antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Annexin V is also know as endonnexin II (ENX2),or placental protein 4 (PP4). It is a member of the family of Ca(2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. Annexin V has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2(basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. It is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 88-102 (SRLYDAYELKHALKG-human) were used as the immunogen for this Annexin V antibody.
Limitation:
This Annexin V antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P08758