Annexin V Antibody

Product Code: NSJ-R31475

Code	Size	Price

NSJ-R31475-100ug

100 ug

£535.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species:

Human
Mouse
Rat

Applications:

Immunohistochemistry- Frozen Section (IHC-F)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

After reconstitution, the Annexin V antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Annexin V antibody and rat samples 1: lung; 2: brain; 3: heart; 4: kidney; and human samples 5: HeLa; 6: SMMC-7721; 7: A549; 8: SGC lysate. Predicted molecular weight ~36 kDa.

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IHC-P: Annexin V antibody testing of human breast cancer tissue

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IHC-P: Annexin V antibody testing of human intestinal cancer tissue

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IHC-P: Annexin V antibody testing of mouse kidney tissue

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IHC-P: Annexin V antibody testing of rat kidney tissue

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IHC staining of frozen human placenta with Annexin V antibody.

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IHC staining of frozen rat kidney with Annexin V antibody.

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Immunofluorescent staining of FFPE human placenta with Annexin V antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

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Immunofluorescent staining of FFPE human U-2 OS cells with Annexin V antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

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Immunofluorescent staining of FFPE rat RH35 cells with Annexin V antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

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Flow cytometry testing of human PC-3 cells with Annexin V antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Annexin V antibody.

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Flow cytometry testing of human SiHa cells with Annexin V antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Annexin V antibody.

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Documents

Further Information

Application Details :

Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells

Application Note:

The stated application concentrations are suggested starting amounts. Titration of the Annexin V antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Description:

Annexin V or A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an Annexin V shield blocks the entry of phospholipids into coagulation (clotting) reactions. Annexin AV can also act as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Format :

Antigen affinity purified

Formulation :

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Gene ID #:

308

Immunogen:

Human partial recombinant protein (AA 2-320) was used as the immunogen for this Annexin V antibody.

Limitation:

This Annexin V antibody is available for research use only.

Purity:

Antigen affinity

Species Reactivity :

Human, Mouse, Rat