Annexin VI Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4425
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4425-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Annexin VI antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 10
Western blot testing of human 1) HeLa, 2) placenta, 3) MCF7, 4) U-87 MG, 5) HepG2, 6) SMMC-7721 and 7) 293T lysate with Annexin VI antibody at 0.5ug/ml. Expected molecular weight: 67-76 kDa.
2 / 10
Western blot testing of 1) rat brain, 2) rat thymus, 3) rat testis, 4) rat pancreas, 5) mouse brain, 6) mouse thymus, 7) mouse testis, 8) mouse pancreas and 9) mouse HEPA1-6 lysate with Annexin VI antibody at 0.5ug/ml. Expected molecular weight: 67-76 kDa.
3 / 10
IHC testing of FFPE human lung cancer tissue with Annexin VI antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 10
IHC testing of FFPE human intestinal cancer tissue with Annexin VI antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 10
IHC testing of FFPE mouse spleen tissue with Annexin VI antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
6 / 10
IHC testing of FFPE rat spleen tissue with Annexin VI antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
7 / 10
Immunofluorescent staining of FFPE human lung cancer with Annexin VI antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
8 / 10
Immunofluorescent staining of FFPE mouse intestine with Annexin VI antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
9 / 10
Immunofluorescent staining of FFPE rat intestine with Annexin VI antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
10 / 10
Flow cytometry testing of human HepG2 cells with Annexin VI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Annexin VI antibody.
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Western blot testing of human 1) HeLa, 2) placenta, 3) MCF7, 4) U-87 MG, 5) HepG2, 6) SMMC-7721 and 7) 293T lysate with Annexin VI antibody at 0.5ug/ml. Expected molecular weight: 67-76 kDa.
Western blot testing of 1) rat brain, 2) rat thymus, 3) rat testis, 4) rat pancreas, 5) mouse brain, 6) mouse thymus, 7) mouse testis, 8) mouse pancreas and 9) mouse HEPA1-6 lysate with Annexin VI antibody at 0.5ug/ml. Expected molecular weight: 67-76 kDa.
IHC testing of FFPE human lung cancer tissue with Annexin VI antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human intestinal cancer tissue with Annexin VI antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse spleen tissue with Annexin VI antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat spleen tissue with Annexin VI antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of FFPE human lung cancer with Annexin VI antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE mouse intestine with Annexin VI antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat intestine with Annexin VI antibody (green) at 1ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human HepG2 cells with Annexin VI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Annexin VI antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml (recombinant human protein) (BSA-free format available)
Application Note:
Optimal dilution of the Annexin VI antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Annexin A6 (ANXA6) is a member of a family of proteins that bind membrane or cytoskeleton in a Ca(2+)-dependent manner. These proteins are characterized by homologous amino acid sequences that are present in multiple copies in each protein. ANXA6 gene is assigned to 5q32-q34 by use of a cDNA clone to probe genomic DNA from rodent-human somatic cell hybrids and for in situ hybridization. The ANX6 gene is approximately 60 kb long and contains 26 exons. The genomic sequence at the 3-prime end does not contain a canonical polyadenylylation signal. Ca(2+)-dependent binding between CRHSP28 and ANXA6 is required for acinar cell membrane trafficking events and digestive enzyme secretion.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human partial recombinant protein corresponding to amino acids N395-L665 was used as the immunogen for the Annexin VI antibody.
Limitation:
This Annexin VI antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P08133