Annexin VII Antibody

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NSJ Bioreagents
Product Code: NSJ-R31187
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31187-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Annexin VII antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE human A431 cells with Annexin VII antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC staining of FFPE human breast cancer tissue with Annexin VII antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Western blot testing of human 1) HeLa, 2) A549, 3) SMMC-7721 and 4) HT1080 cell lysate with Annexin VII antibody. Predicted molecular weight ~52 kDa.
4 / 5
Western blot testing of human 1) SMMC-7721 and 2) COLO-320 cell lysate with Annexin VII antibody. Predicted molecular weight ~52 kDa.
5 / 5
Flow cytometry testing of human HepG2 cells with Annexin VII antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Annexin VII antibody.
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Immunofluorescent staining of FFPE human A431 cells with Annexin VII antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human breast cancer tissue with Annexin VII antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) A549, 3) SMMC-7721 and 4) HT1080 cell lysate with Annexin VII antibody. Predicted molecular weight ~52 kDa.
Western blot testing of human 1) SMMC-7721 and 2) COLO-320 cell lysate with Annexin VII antibody. Predicted molecular weight ~52 kDa.
Flow cytometry testing of human HepG2 cells with Annexin VII antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Annexin VII antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Annexin VII antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Annexin A7, also known as ANX7 or SNX, is a protein that in humans is encoded by the ANXA7 gene. Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins. Shirvan et al.(1994) isolated genomic clones of the ANXA7 gene and assigned the gene to 10q21.1-q21.2 by study of somatic cell hybrids and by in situ hybridization. Srivastava et al.(2001) concluded that the gene exhibits many biologic and genetic properties expected of a tumor suppressor gene and may play a role in prostate cancer progression. Caohuy et al.(1996) present experimental evidence, based on studies of recombinant human protein and isolated bovine chromaffin cells, that ANXA7 is a Ca(2+)-dependent GTP binding protein. It was active in a chromaffin granule aggregation assays in the presence of Ca(2+) and GTP, and was deactivated upon GTP hydrolysis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Annexin VII (TRSEIDLVQIKQMFAQ) was used as the immunogen for this Annexin VII antibody.
Limitation:
This Annexin VII antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P20073