APE1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31549
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31549-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the APE1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of APE1 antibody and Lane 1: rat NRK; 2: human HeLa; 3: (r) PC12; 4: (r) RH35; 5: mouse HEPA; 6: (h) MCF7: (h) A549; 8: (h) placenta; 9: (h) A431. Expected/observed size ~38KD
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Western blot testing of APE1 antibody and recombinant human protein (0.5ng)
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IHC-P: APE1 antibody testing of human lung cancer tissue
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IHC-P staining of mouse brain tissue
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IHC-P staining of rat brain tissue
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IF/ICC staining of FFPE human U-2 OS cells with APE1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human U937 cells with APE1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= APE1 antibody.
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Immunofluorescent staining of FFPE human colon cancer tissue with APE1 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of APE1 antibody and Lane 1: rat NRK; 2: human HeLa; 3: (r) PC12; 4: (r) RH35; 5: mouse HEPA; 6: (h) MCF7: (h) A549; 8: (h) placenta; 9: (h) A431. Expected/observed size ~38KD
Western blot testing of APE1 antibody and recombinant human protein (0.5ng)
IHC-P: APE1 antibody testing of human lung cancer tissue
IHC-P staining of mouse brain tissue
IHC-P staining of rat brain tissue
IF/ICC staining of FFPE human U-2 OS cells with APE1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U937 cells with APE1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= APE1 antibody.
Immunofluorescent staining of FFPE human colon cancer tissue with APE1 antibody. HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence/Immunocytochemistry (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the APE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime, 5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the gene is mapped to 14q11.2-q12. The predicted protein, which contains probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APE1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APE1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
328
Immunogen:
Human partial recombinant protein (AA 2-318) was used as the immunogen for this APE1 antibody.
Limitation:
This APE1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat