APOA1 Antibody / Apolipoprotein A I

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NSJ Bioreagents
Product Code: NSJ-R32382
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32382-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the APOA1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of human placenta lysate with APOA1 antibody. Predicted molecular weight ~30 kDa.
2 / 5
IHC testing of FFPE human liver cancer tissue with APOA1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
3 / 5
IHC testing of FFPE human kidney cancer tissue with APOA1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
4 / 5
Flow cytometry testing of human Caco-2 cells with APOA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=APOA1 antibody.
5 / 5
Flow cytometry testing of human HepG2 cells with APOA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=APOA1 antibody.
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Western blot testing of human placenta lysate with APOA1 antibody. Predicted molecular weight ~30 kDa.
IHC testing of FFPE human liver cancer tissue with APOA1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE human kidney cancer tissue with APOA1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
Flow cytometry testing of human Caco-2 cells with APOA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=APOA1 antibody.
Flow cytometry testing of human HepG2 cells with APOA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=APOA1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,ELISA: 0.1-0.5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the APOA1 antibody should be determined by the researcher.
Description:
Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It binds to lipopolysaccharide or endotoxin, and has a major role in the anti-endotoxin function of HDL.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids D25-Q267 of the human protein were used as the immunogen for the APOA1 antibody.
Limitation:
This APOA1 antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P02647