APOBEC3G Antibody

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NSJ Bioreagents
Product Code: NSJ-R32470
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32470-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Prior to reconstitution, store at 40. After reconstitution, the APOBEC3G antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human A431 and 2) human Jurkat cell lysate with APOBEC3G antibody at 0.5ug/ml. Predicted molecular weight: ~46 kDa.
2 / 4
IHC testing of FFPE human pancreatic cancer tissue with APOBEC3G antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
3 / 4
IHC testing of FFPE human tonsil with APOBEC3G antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human THP-1 cells with APOBEC3G antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=APOBEC3G antibody.
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Western blot testing of 1) human A431 and 2) human Jurkat cell lysate with APOBEC3G antibody at 0.5ug/ml. Predicted molecular weight: ~46 kDa.
IHC testing of FFPE human pancreatic cancer tissue with APOBEC3G antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
IHC testing of FFPE human tonsil with APOBEC3G antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
Flow cytometry testing of human THP-1 cells with APOBEC3G antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=APOBEC3G antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
APOBEC3G (Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G) is a human enzyme encoded by the APOBEC3G gene. This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids E191-N384 were used as the immunogen for the APOBEC3G antibody.
Limitation:
This APOBEC3G antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q9HC16