ApoE Antibody / Apolipoprotein E

Contact us
NSJ Bioreagents
Product Code: NSJ-R32471
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32471-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Prior to reconstitution, store at 40. After reconstitution, the Apolipoprotein E antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Immunofluorescent staining of FFPE rat liver tissue with Apolipoprotein E antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 6
Immunofluorescent staining of FFPE mouse liver tissue with Apolipoprotein E antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 6
Immunofluorescent staining of FFPE mouse HEPA1-6 cells with Apolipoprotein E antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 6
IHC testing of FFPE rat liver with APOE antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
5 / 6
IHC testing of FFPE mouse liver with APOE antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
6 / 6
Western blot testing of 1) mouse spleen and 2) mouse kidney lysate with APOE antibody at 0.5ug/ml. Predicted molecular weight: 34-37 kDa.
❮❯

Immunofluorescent staining of FFPE rat liver tissue with Apolipoprotein E antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE mouse liver tissue with Apolipoprotein E antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE mouse HEPA1-6 cells with Apolipoprotein E antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat liver with APOE antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
IHC testing of FFPE mouse liver with APOE antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
Western blot testing of 1) mouse spleen and 2) mouse kidney lysate with APOE antibody at 0.5ug/ml. Predicted molecular weight: 34-37 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
ApoE is also known as AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids D55-Q294 were used as the immunogen for the Apolipoprotein E antibody.
Limitation:
This Apolipoprotein E antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
P08226