Apoptosis inhibitor 5 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R30177
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30177-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Apoptosis inhibitor 5 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 8
IHC staining of FFPE human placental tissue with Apoptosis inhibitor 5 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
2 / 8
IHC staining of FFPE rat intestine tissue with Apoptosis inhibitor 5 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
3 / 8
IHC staining of FFPE mouse intestine tissue with Apoptosis inhibitor 5 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE rat brain tissue with Apoptosis inhibitor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of frozen rat heart tissue with Apoptosis inhibitor 5 antibody.
6 / 8
ICC staining of human HeLa cells with Apoptosis inhibitor 5 antibody.
7 / 8
Immunofluorescent staining of FFPE human SK-O-V3 cells with Apoptosis inhibitor 5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
8 / 8
Western blot testing of Apoptosis Inhibitor 5 antibody and Lane 1: rat heart; 2: rat brain; 3: rat testis; 4: rat placenta; 5: human MCF-7; 6: human HeLa; 7: human CEM; 8: human SMMC-7721; 9: human COLO320 cell lysate. Predicted molecular weight ~59 kDa.
❮❯

IHC staining of FFPE human placental tissue with Apoptosis inhibitor 5 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine tissue with Apoptosis inhibitor 5 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE mouse intestine tissue with Apoptosis inhibitor 5 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Apoptosis inhibitor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of frozen rat heart tissue with Apoptosis inhibitor 5 antibody.
ICC staining of human HeLa cells with Apoptosis inhibitor 5 antibody.
Immunofluorescent staining of FFPE human SK-O-V3 cells with Apoptosis inhibitor 5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of Apoptosis Inhibitor 5 antibody and Lane 1: rat heart; 2: rat brain; 3: rat testis; 4: rat placenta; 5: human MCF-7; 6: human HeLa; 7: human CEM; 8: human SMMC-7721; 9: human COLO320 cell lysate. Predicted molecular weight ~59 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Apoptosis inhibitor 5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death(apoptosis). Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. The API5 gene to chromosome 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Apoptosis inhibitor 5 (amino acids 487-504 of isoform b, KYSSNLGNFNYERSLQGK) was used as the immunogen for this Apoptosis inhibitor 5 antibody.
Limitation:
This Apoptosis inhibitor 5 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9BZZ5