Apoptosis-Inducing Factor Antibody (Mitochondrial marker)

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NSJ Bioreagents
Product Code: NSJ-R31356
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31356-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Apoptosis-Inducing Factor antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Apoptosis-Inducing Factor antibody and Lane 1: rat heart; 2: (r) brain; 3: human K562; 4: (h) HEPG2; 5: (h) A431; 6: mouse NIH3T3 cell lysate. Expected/observed size ~67KD
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IHC-P: Apoptosis-Inducing Factor antibody testing of human lung cancer tissue
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IHC-P: Apoptosis-Inducing Factor antibody testing of rat heart tissue
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Western blot testing of Apoptosis-Inducing Factor antibody and Lane 1: rat heart; 2: (r) brain; 3: human K562; 4: (h) HEPG2; 5: (h) A431; 6: mouse NIH3T3 cell lysate. Expected/observed size ~67KD
IHC-P: Apoptosis-Inducing Factor antibody testing of human lung cancer tissue
IHC-P: Apoptosis-Inducing Factor antibody testing of rat heart tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Apoptosis-Inducing Factor antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. A related pseudogene has been identified on chromosome 10.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human AIFM1 (EQHEDLNEVAKLFNIHED) was used as the immunogen for this Apoptosis-Inducing Factor antibody (100% homologous in human, mouse and rat).
Limitation:
This Apoptosis-Inducing Factor antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O95831