ARA9 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31473
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31473-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
After reconstitution, the ARA9 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-2 OS cells with ARA9 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human A431 cells with ARA9 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ARA9 antibody.
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Western blot testing of ARA9 antibody and Lane 1: HeLa; 2: COLO320; 3: HT1080; 4: MCF-7; 5: SW620; 6: U87; 7: MM231; Predicted/Observed size: 37KD
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Immunofluorescent staining of FFPE human U-2 OS cells with ARA9 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A431 cells with ARA9 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ARA9 antibody.
Western blot testing of ARA9 antibody and Lane 1: HeLa; 2: COLO320; 3: HT1080; 4: MCF-7; 5: SW620; 6: U87; 7: MM231; Predicted/Observed size: 37KD

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the ARA9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
aryl hydrocarbon receptor-associated protein-9 (ARA9), also known as AIP or XAP-2, is a protein that in humans is encoded by the AIP gene. This gene is mapped to 11q13.2. The encoded protein is found in the cytoplasm as part of a multiprotein complex, but upon binding of ligand is transported to the nucleus. ARA9/AIP may play a positive role in aryl hydrocarbon receptor-mediated signalling possibly by influencing its receptivity for ligand and/or its nuclear targeting. It has been shown that ARA9 is the cellular negative regulator of the hepatitis B virus (HBV) X protein. Mutations may be the cause of a familial form of acromegaly, familial isolated pituitary adenoma (FIPA).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
9049
Immunogen:
Human partial recombinant protein (AA 91-330) was used as the immunogen for this ARA9 antibody.
Limitation:
This ARA9 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human